abstract：:Ischemic stroke enhances the proliferation of adult-generated precursor cells that ectopically migrate toward the infarct. Studies have correlated precursor cell proliferation and subsequent adult neurogenesis with enhanced stroke recovery, yet it remains unclear whether stroke can generate new neurons capable of func...

journal_title：Stem cell reports

authors： Kannangara TS,Carter A,Xue Y,Dhaliwal JS,Béïque JC,Lagace DC

更新日期：2018-12-11 00:00:00

abstract：:The small intestine plays an important role in the absorption and metabolism of oral drugs. In the current evaluation system, it is difficult to predict the precise absorption and metabolism of oral drugs. In this study, we generated small intestinal epithelial-like cells from human induced pluripotent stem cells (hiP...

journal_title：Stem cell reports

authors： Negoro R,Takayama K,Kawai K,Harada K,Sakurai F,Hirata K,Mizuguchi H

更新日期：2018-12-11 00:00:00

abstract：:Being able to summarize properly your work is not an easy task. But learning the skill of writing a good abstract is very important, as it can open many doors, including the possibility to be selected as a speaker at conferences. As meeting abstract reviewers, here we are writing to give you insights into the abstract...

journal_title：Stem cell reports

authors： Sturgeon CM,Ditadi A

更新日期：2018-12-11 00:00:00

abstract：:Hematopoietic stem cells (HSCs) ensure a life-long regeneration of the blood system and are therefore an important source for transplantation and gene therapy. The teratoma environment supports the complex development of functional HSCs from human pluripotent stem cells, which is difficult to recapitulate in culture. ...

journal_title：Stem cell reports

authors： Philipp F,Selich A,Rothe M,Hoffmann D,Rittinghausen S,Morgan MA,Klatt D,Glage S,Lienenklaus S,Neuhaus V,Sewald K,Braun A,Schambach A

更新日期：2018-11-13 00:00:00

abstract：:Fanconi anemia (FA) causes bone marrow failure early during childhood, and recent studies indicate that a hematopoietic defect could begin in utero. We performed a unique kinetics study of hematopoiesis in Fancg-/- mouse embryos, between the early embryonic day 11.5 (E11.5) to E12.5 developmental window (when the high...

journal_title：Stem cell reports

authors： Domenech C,Maillard L,Rousseau A,Guidez F,Petit L,Pla M,Clay D,Guimiot F,Sanfilippo S,Jacques S,de la Grange P,Robil N,Soulier J,Souyri M

更新日期：2018-11-13 00:00:00

abstract：:Several studies have reported an association between the farnesoid X receptor alpha (FXRα) and estrogenic signaling pathways. Fxrα could thus be involved in the reprotoxic effects of endocrine disruptors such as bisphenol-A (BPA). To test this hypothesis, mice were exposed to BPA and/or stigmasterol (S), an FXRα antag...

journal_title：Stem cell reports

authors： Sèdes L,Desdoits-Lethimonier C,Rouaisnel B,Holota H,Thirouard L,Lesne L,Damon-Soubeyrand C,Martinot E,Saru JP,Mazaud-Guittot S,Caira F,Beaudoin C,Jégou B,Volle DH

更新日期：2018-10-09 00:00:00

abstract：:Understanding the cellular properties controlling neural stem and progenitor cell (NSPC) fate choice will improve their therapeutic potential. The electrophysiological measure whole-cell membrane capacitance reflects fate bias in the neural lineage but the cellular properties underlying membrane capacitance are poorly...

journal_title：Stem cell reports

authors： Yale AR,Nourse JL,Lee KR,Ahmed SN,Arulmoli J,Jiang AYL,McDonnell LP,Botten GA,Lee AP,Monuki ES,Demetriou M,Flanagan LA

更新日期：2018-10-09 00:00:00

abstract：:Pelizaeus-Merzbacher disease (PMD) is a fatal X-linked disorder caused by loss of myelinating oligodendrocytes and consequent hypomyelination. The underlying cellular and molecular dysfunctions are not fully defined, but therapeutic enhancement of oligodendrocyte survival could restore functional myelination in patien...

journal_title：Stem cell reports

authors： Elitt MS,Shick HE,Madhavan M,Allan KC,Clayton BLL,Weng C,Miller TE,Factor DC,Barbar L,Nawash BS,Nevin ZS,Lager AM,Li Y,Jin F,Adams DJ,Tesar PJ

更新日期：2018-09-11 00:00:00

abstract：:Although the functional roles of long noncoding RNAs (lncRNAs) have been increasingly identified, few lncRNAs that control the naïve state of embryonic stem cells (ESCs) are known. Here, we report a naïve-state-associated lncRNA, LincU, which is intrinsically activated by Nanog in mESCs. LincU-deficient mESCs exhibit ...

journal_title：Stem cell reports

authors： Jiapaer Z,Li G,Ye D,Bai M,Li J,Guo X,Du Y,Su D,Jia W,Chen W,Wang G,Yu Y,Zhu F,Wan X,Kang J

更新日期：2018-08-14 00:00:00

abstract：:We have developed an improved episomal vector system for efficient generation of integration-free induced pluripotent stem cells (iPSCs) from peripheral blood mononuclear cells. More recently, we reported that the use of an optimized CRISPR-Cas9 system together with a double-cut donor increases homology-directed repai...

journal_title：Stem cell reports

authors： Wen W,Cheng X,Fu Y,Meng F,Zhang JP,Zhang L,Li XL,Yang Z,Xu J,Zhang F,Botimer GD,Yuan W,Sun C,Cheng T,Zhang XB

更新日期：2018-06-05 00:00:00

abstract：:Both glial cell line-derived neurotrophic factor (GDNF) and fibroblast growth factor 2 (FGF2) are bona fide self-renewal factors for spermatogonial stem cells, whereas retinoic acid (RA) induces spermatogonial differentiation. In this study, we investigated the functional differences between FGF2 and GDNF in the germl...

journal_title：Stem cell reports

authors： Masaki K,Sakai M,Kuroki S,Jo JI,Hoshina K,Fujimori Y,Oka K,Amano T,Yamanaka T,Tachibana M,Tabata Y,Shiozawa T,Ishizuka O,Hochi S,Takashima S

更新日期：2018-06-05 00:00:00

abstract：:Nakajo-Nishimura syndrome (NNS) is an immunoproteasome-associated autoinflammatory disorder caused by a mutation of the PSMB8 gene. Although dysfunction of the immunoproteasome causes various cellular stresses attributed to the overproduction of inflammatory cytokines and chemokines in NNS, the underlying mechanisms o...

journal_title：Stem cell reports

authors： Honda-Ozaki F,Terashima M,Niwa A,Saiki N,Kawasaki Y,Ito H,Hotta A,Nagahashi A,Igura K,Asaka I,Li HL,Yanagimachi M,Furukawa F,Kanazawa N,Nakahata T,Saito MK

更新日期：2018-06-05 00:00:00

abstract：:Thyroid hormone (TH) transporters are required for the transmembrane passage of TH in target cells. In humans, inactivating mutations in the TH transporter MCT8 cause the Allan-Herndon-Dudley syndrome, characterized by severe neuromuscular symptoms and an abnormal TH serum profile, which is fully replicated in Mct8 kn...

journal_title：Stem cell reports

authors： Mayerl S,Schmidt M,Doycheva D,Darras VM,Hüttner SS,Boelen A,Visser TJ,Kaether C,Heuer H,von Maltzahn J

更新日期：2018-06-05 00:00:00

abstract：:Following Jamie Thomson's lecture on primate embryonic stem cells (ESCs) at a meeting I had organized in March 1997, in Israel, to celebrate receipt of the Wolf Prize in Agriculture to my colleague and friend Neal First, frozen human embryos donated for research in Israel were shipped to Wisconsin. The five hESC lines...

journal_title：Stem cell reports

authors： Itskovitz-Eldor J

更新日期：2018-05-08 00:00:00

abstract：:The first hematopoietic stem and progenitor cells are generated during development from hemogenic endothelium (HE) through trans-differentiation. The molecular mechanisms underlying this endothelial-to-hematopoietic transition (EHT) remain poorly understood. Here, we explored the role of the epigenetic regulators HDAC...

journal_title：Stem cell reports

authors： Thambyrajah R,Fadlullah MZH,Proffitt M,Patel R,Cowley SM,Kouskoff V,Lacaud G

更新日期：2018-04-10 00:00:00

abstract：:Human stem cell-derived models of development and neurodegenerative diseases are challenged by cellular immaturity in vitro. Microengineered organ-on-chip (or Organ-Chip) systems are designed to emulate microvolume cytoarchitecture and enable co-culture of distinct cell types. Brain microvascular endothelial cells (BM...

journal_title：Stem cell reports

authors： Sances S,Ho R,Vatine G,West D,Laperle A,Meyer A,Godoy M,Kay PS,Mandefro B,Hatata S,Hinojosa C,Wen N,Sareen D,Hamilton GA,Svendsen CN

更新日期：2018-04-10 00:00:00

abstract：:The balance between factors leading to proliferation and differentiation of cortical neural precursors (CNPs) determines the correct cortical development. In this work, we show that GDNF and its receptor GFRα1 are expressed in the neocortex during the period of cortical neurogenesis. We show that the GDNF/GFRα1 comple...

journal_title：Stem cell reports

authors： Bonafina A,Fontanet PA,Paratcha G,Ledda F

更新日期：2018-03-13 00:00:00

abstract：:The transition from naive to primed state of pluripotent stem cells is hallmarked by epithelial-mesenchymal transition, metabolic switch from oxidative phosphorylation to aerobic glycolysis, and changes in the epigenetic landscape. Since these changes are also seen as putative hallmarks of neoplastic cell transformati...

journal_title：Stem cell reports

authors： Altshuler A,Verbuk M,Bhattacharya S,Abramovich I,Haklai R,Hanna JH,Kloog Y,Gottlieb E,Shalom-Feuerstein R

更新日期：2018-03-13 00:00:00

abstract：:Developing effective therapeutics for complex diseases such as late-onset, sporadic Alzheimer's disease (SAD) is difficult due to genetic and environmental heterogeneity in the human population and the limitations of existing animal models. Here, we used hiPSC-derived neurons to test a compound that stabilizes the ret...

journal_title：Stem cell reports

authors： Young JE,Fong LK,Frankowski H,Petsko GA,Small SA,Goldstein LSB

更新日期：2018-03-13 00:00:00

abstract：:Properties of cancer stem cells involved in drug resistance and relapse have significant effects on clinical outcome. Although tyrosine kinase inhibitors (TKIs) have dramatically improved survival of patients with chronic myeloid leukemia (CML), TKIs have not fully cured CML due to TKI-resistant CML stem cells. Moreov...

journal_title：Stem cell reports

authors： Miyauchi M,Koya J,Arai S,Yamazaki S,Honda A,Kataoka K,Yoshimi A,Taoka K,Kumano K,Kurokawa M

更新日期：2018-03-13 00:00:00

abstract：:Non-human primates (NHPs) can serve as a human-like model to study cell therapy using induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs). However, whether the efficacy of NHP and human iPSC-CMs is mechanistically similar remains unknown. To examine this, RNU rats received intramyocardial injection of 1 × ...

journal_title：Stem cell reports

authors： Zhao X,Chen H,Xiao D,Yang H,Itzhaki I,Qin X,Chour T,Aguirre A,Lehmann K,Kim Y,Shukla P,Holmström A,Zhang JZ,Zhuge Y,Ndoye BC,Zhao M,Neofytou E,Zimmermann WH,Jain M,Wu JC

更新日期：2018-02-13 00:00:00

abstract：:Human vision relies heavily upon cone photoreceptors, and their loss results in permanent visual impairment. Transplantation of healthy photoreceptors can restore visual function in models of inherited blindness, a process previously understood to arise by donor cell integration within the host retina. However, we and...

journal_title：Stem cell reports

authors： Waldron PV,Di Marco F,Kruczek K,Ribeiro J,Graca AB,Hippert C,Aghaizu ND,Kalargyrou AA,Barber AC,Grimaldi G,Duran Y,Blackford SJI,Kloc M,Goh D,Zabala Aldunate E,Sampson RD,Bainbridge JWB,Smith AJ,Gonzalez-Cordero A,S

更新日期：2018-02-13 00:00:00

abstract：:Cellular replacement therapies for neurological conditions use human embryonic stem cell (hESC)- or induced pluripotent stem cell (hiPSC)-derived neurons to replace damaged or diseased populations of neurons. For the spinal cord, significant progress has been made generating the in-vitro-derived motor neurons required...

journal_title：Stem cell reports

authors： Gupta S,Sivalingam D,Hain S,Makkar C,Sosa E,Clark A,Butler SJ

更新日期：2018-02-13 00:00:00

abstract：:Unambiguous cell line authentication is essential to avoid loss of association between data and cells. The risk for loss of references increases with the rapidity that new human pluripotent stem cell (hPSC) lines are generated, exchanged, and implemented. Ideally, a single name should be used as a generally applied re...

journal_title：Stem cell reports

authors： Kurtz A,Seltmann S,Bairoch A,Bittner MS,Bruce K,Capes-Davis A,Clarke L,Crook JM,Daheron L,Dewender J,Faulconbridge A,Fujibuchi W,Gutteridge A,Hei DJ,Kim YO,Kim JH,Kokocinski AK,Lekschas F,Lomax GP,Loring JF,Ludwig

更新日期：2018-01-09 00:00:00

abstract：:Alzheimer's disease (AD) induces memory and cognitive impairment in the absence of motor and sensory deficits during its early and middle course. A major unresolved question is the basis for this selective neuronal vulnerability. Aβ, which plays a central role in AD pathogenesis, is generated throughout the brain, yet...

journal_title：Stem cell reports

authors： Muratore CR,Zhou C,Liao M,Fernandez MA,Taylor WM,Lagomarsino VN,Pearse RV 2nd,Rice HC,Negri JM,He A,Srikanth P,Callahan DG,Shin T,Zhou M,Bennett DA,Noggle S,Love JC,Selkoe DJ,Young-Pearse TL

更新日期：2017-12-12 00:00:00

abstract：:High-purity cardiomyocytes (CMs) derived from human induced pluripotent stem cells (hiPSCs) are promising for drug development and myocardial regeneration. However, most hiPSC-derived CMs morphologically and functionally resemble immature rather than adult CMs, which could hamper their application. Here, we obtained h...

journal_title：Stem cell reports

authors： Li J,Minami I,Shiozaki M,Yu L,Yajima S,Miyagawa S,Shiba Y,Morone N,Fukushima S,Yoshioka M,Li S,Qiao J,Li X,Wang L,Kotera H,Nakatsuji N,Sawa Y,Chen Y,Liu L

更新日期：2017-11-14 00:00:00

abstract：:Cardiac regenerative therapies utilizing human induced pluripotent stem cells (hiPSCs) are hampered by ineffective large-scale culture. hiPSCs were cultured in multilayer culture plates (CPs) with active gas ventilation (AGV), resulting in stable proliferation and pluripotency. Seeding of 1 × 106 hiPSCs per layer yiel...

journal_title：Stem cell reports

authors： Tohyama S,Fujita J,Fujita C,Yamaguchi M,Kanaami S,Ohno R,Sakamoto K,Kodama M,Kurokawa J,Kanazawa H,Seki T,Kishino Y,Okada M,Nakajima K,Tanosaki S,Someya S,Hirano A,Kawaguchi S,Kobayashi E,Fukuda K

更新日期：2017-11-14 00:00:00

abstract：:We identified osteoclast defects in craniometaphyseal dysplasia (CMD) using an easy-to-use protocol for differentiating osteoclasts from human induced pluripotent stem cells (hiPSCs). CMD is a rare genetic bone disorder, characterized by life-long progressive thickening of craniofacial bones and abnormal shape of long...

journal_title：Stem cell reports

authors： Chen IP,Luxmi R,Kanaujiya J,Hao Z,Reichenberger EJ

更新日期：2017-11-14 00:00:00

abstract：:Antibody-mediated rejection is characterized by donor-specific antibody produced by B cells. However, to our knowledge, B cell invasion and antibody in the inflamed retina after transplantation of retinal pigment epithelial (RPE) cells has not been reported. To determine if RPE transplantation could be performed using...

journal_title：Stem cell reports

authors： Sugita S,Makabe K,Fujii S,Iwasaki Y,Kamao H,Shiina T,Ogasawara K,Takahashi M

更新日期：2017-11-14 00:00:00

abstract：:Molecular mechanisms responsible for the development of human skin epithelial cells are incompletely understood. As a consequence, the efficiency to establish a pure skin epithelial cell population from human induced pluripotent stem cells (hiPSCs) remains poor. Using an approach including RNAi and high-throughput ima...

journal_title：Stem cell reports

authors： Larribère L,Galach M,Novak D,Arévalo K,Volz HC,Stark HJ,Boukamp P,Boutros M,Utikal J

更新日期：2017-10-10 00:00:00

abstract：:Generation of hematopoietic stem cells (HSCs) from pluripotent stem cells (PSCs) could potentially provide unlimited HSCs for clinical transplantation, a curative treatment for numerous blood diseases. However, to date, bona fide HSC generation has been largely unsuccessful in vitro. We have previously described proof...

journal_title：Stem cell reports

authors： Tsukada M,Ota Y,Wilkinson AC,Becker HJ,Osato M,Nakauchi H,Yamazaki S

更新日期：2017-10-10 00:00:00

abstract：:Pluripotent cells emanate from the inner cell mass (ICM) of the blastocyst and when cultivated under optimal conditions immortalize as embryonic stem cells (ESCs). The fundamental mechanism underlying ESC derivation has, however, remained elusive. Recently, we have devised a highly efficient approach for establishing ...

journal_title：Stem cell reports

authors： Totonchi M,Hassani SN,Sharifi-Zarchi A,Tapia N,Adachi K,Arand J,Greber B,Sabour D,Araúzo-Bravo MJ,Walter J,Pakzad M,Gourabi H,Schöler HR,Baharvand H

更新日期：2017-10-10 00:00:00

abstract：:The hematopoietic marrow microenvironment is composed of multiple cell types embedded in an extracellular matrix (ECM). We have explored marrow ECM using mass spectrometry and found dermatopontin (DPT), a small non-collagenous ECM protein, to be present. We found that DPT cooperates with other ECM proteins to promote ...

journal_title：Stem cell reports

authors： Kramer AC,Blake AL,Taisto ME,Lehrke MJ,Webber BR,Lund TC

更新日期：2017-09-12 00:00:00

abstract：:The Polycomb group gene BMI1 is essential for efficient muscle regeneration in a mouse model of Duchenne muscular dystrophy, and its enhanced expression in adult skeletal muscle satellite cells ameliorates the muscle strength in this model. Here, we show that the impact of mild BMI1 overexpression observed in mouse mo...

journal_title：Stem cell reports

authors： Dibenedetto S,Niklison-Chirou M,Cabrera CP,Ellis M,Robson LG,Knopp P,Tedesco FS,Ragazzi M,Di Foggia V,Barnes MR,Radunovic A,Marino S

更新日期：2017-08-08 00:00:00

abstract：:Understanding the processes that govern liver progenitor cell differentiation has important implications for the design of strategies targeting chronic liver diseases, whereby regeneration of liver tissue is critical. Although DNA methylation (5mC) and hydroxymethylation (5hmC) are highly dynamic during early embryoni...

journal_title：Stem cell reports

authors： Ancey PB,Ecsedi S,Lambert MP,Talukdar FR,Cros MP,Glaise D,Narvaez DM,Chauvet V,Herceg Z,Corlu A,Hernandez-Vargas H

更新日期：2017-07-11 00:00:00

abstract：:BACE1 is validated as Alzheimer's β-secretase and a therapeutic target for Alzheimer's disease. In examining BACE1-null mice, we discovered that BACE1 deficiency develops abnormal clusters of immature neurons, forming doublecortin-positive neuroblasts, in the developing dentate gyrus, mainly in the subpial zone (SPZ)....

journal_title：Stem cell reports

authors： Hou H,Fan Q,He W,Suh H,Hu X,Yan R

更新日期：2017-07-11 00:00:00

abstract：:Pluripotent stem cells have been proposed as an unlimited source of pancreatic β cells for studying and treating diabetes. However, the long, multi-step differentiation protocols used to generate functional β cells inevitably exhibit considerable variability, particularly when applied to pluripotent cells from diverse...

journal_title：Stem cell reports

authors： Trott J,Tan EK,Ong S,Titmarsh DM,Denil SLIJ,Giam M,Wong CK,Wang J,Shboul M,Eio M,Cooper-White J,Cool SM,Rancati G,Stanton LW,Reversade B,Dunn NR

更新日期：2017-06-06 00:00:00

abstract：:Microglia, the immune cells of the brain, are crucial to proper development and maintenance of the CNS, and their involvement in numerous neurological disorders is increasingly being recognized. To improve our understanding of human microglial biology, we devised a chemically defined protocol to generate human microgl...

journal_title：Stem cell reports

authors： Douvaras P,Sun B,Wang M,Kruglikov I,Lallos G,Zimmer M,Terrenoire C,Zhang B,Gandy S,Schadt E,Freytes DO,Noggle S,Fossati V

更新日期：2017-06-06 00:00:00

abstract：:The core pluripotency transcription factor NANOG is critical for embryonic stem cell (ESC) self-renewal and somatic cell reprogramming. Although NANOG is phosphorylated at multiple residues, the role of NANOG phosphorylation in ESC self-renewal is incompletely understood, and no information exists regarding its functi...

journal_title：Stem cell reports

authors： Saunders A,Li D,Faiola F,Huang X,Fidalgo M,Guallar D,Ding J,Yang F,Xu Y,Zhou H,Wang J

更新日期：2017-05-09 00:00:00

abstract：:Characterization of non-neoplastic and malignant human stem cell populations in their native state can provide new insights into gliomagenesis. Here we developed a purification strategy to directly isolate EGFR+/- populations from human germinal matrix (GM) and adult subventricular zone autopsy tissues, and from de no...

journal_title：Stem cell reports

authors： Tome-Garcia J,Tejero R,Nudelman G,Yong RL,Sebra R,Wang H,Fowkes M,Magid M,Walsh M,Silva-Vargas V,Zaslavsky E,Friedel RH,Doetsch F,Tsankova NM

更新日期：2017-05-09 00:00:00